Open AccessPhosphonoacetic Acid-Resistant Mutants of Herpes Simplex Virus: Effect of Phosphonoacetic Acid on Virus Replication and In Vitro Deoxyribonucleic Acid Synthesis in Isolated Nuclei
Author(s) -
Yechiel Becker,
Yael Asher,
Yaffa Cohen,
Eynat Weinberg-Zahlering,
Joseph Shlomai
Publication year - 1977
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.11.5.919
Subject(s) - herpes simplex virus , mutant , in vitro , bromodeoxyuridine , dna , dna replication , virus , thymidine , dna synthesis , viral replication , dna polymerase , chemistry , microbiology and biotechnology , virology , biology , biochemistry , gene , cell growth
Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 104 plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.