Open AccessInducible Resistance to d -Cycloserine in Bacillus subtilis 168
Author(s) -
Virginia L. Clark,
Frank E. Young
Publication year - 1977
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.11.5.871
Subject(s) - cycloserine , bacillus subtilis , glycine , microbiology and biotechnology , peptidoglycan , biology , antibiotics , chemistry , bacteria , biochemistry , enzyme , amino acid , genetics
Resistance tod -cycloserine could be induced inBacillus subtilis 168 by sublethal concentrations ofd -cycloserine. Sensitivity to the antibiotic could be regained by growth in the absence ofd -cycloserine. The bactericidal activity ofd -cycloserine apparently was not altered by resistant cells, and peptidoglycan synthesis was still inhibited byd -cycloserine in resistant cells. Thed -cycloserine resistance apparently resulted from a decreased uptake of the antibiotic. The decrease ind -cycloserine transport could be prevented by simultaneous treatment of the cells with rifampin andd -cycloserine.d -Cycloserine was transported by the same system as glycine inB. subtilis .d -Cycloserine was able to exchange for intracellular glycine in both sensitive and resistant cells, suggesting thatd -cycloserine is not excluded from the cell in resistant cultures.