Open AccessAntibacterial Activity of Lividomycin Toward R Factor-Resistant Strains of Escherichia coli
Author(s) -
Masatoshi Yamaguchi,
Fujio Kobayashi,
Susumu Mitsuhashi
Publication year - 1972
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.1.2.139
Subject(s) - escherichia coli , kanamycin , microbiology and biotechnology , alkaline phosphatase , streptomycin , biology , enterobacteriaceae , antibiotics , chemistry , biochemistry , enzyme , gene
Forty-eight R factors conferring resistance to both kanamycin (KM) and streptomycin (SM) were demonstrated from 1,270Escherichia coli strains of clinical origin. Among these R factors isolated, 42 also conferred resistance to the new antibiotic lividomycin (LV). Extracts ofE. coli ML1410 carrying one such factor (RM81 ) inactivated LV as well as KM and SM. But extracts ofE. coli ML1410 carrying an R factor (RM82 or RM83 ) sensitive to LV could not inactivate LV. LV inactivation required both adenosinetriphosphate (ATP) and Mg2+ . Inactivated LV was reactivable by alkaline phosphatase. Isotopic studies with labeled ATP and elemental analysis of the reaction product indicate that it is a monophosphorylated derivative of LV.