Open AccessTransferable Drug Resistance in Pseudomonas aeruginosa
Author(s) -
L. E. Bryan,
H. M. Van Den Elzen,
Jui Teng Tseng
Publication year - 1972
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.1.1.22
Subject(s) - pseudomonas aeruginosa , streptomycin , escherichia coli , microbiology and biotechnology , strain (injury) , biology , pseudomonadaceae , drug resistance , pseudomonadales , pseudomonas , bacteria , antibiotics , genetics , gene , anatomy
Three strains ofPseudomonas aeruginosa were demonstrated to transfer double-drug resistance by conjugation to aP. aeruginosa recipient at frequencies of 10−4 to 10−2 per recipient cell. Two of the three strains also transferred toEscherichia coli at frequencies which were 103 - to 105 -fold lower, but the third strain could not be demonstrated to do so. The latter strain, however, conferred maleness on thePseudomonas recipient. The transfer of streptomycin resistance was associated with the acquisition of streptomycin phosphorylase by bothP. aeruginosa andE. coli recipients. Maximal broth mating frequencies were obtained with nonagitated cultures less than 1 mm in depth. A pyocine selection system based on donor sensitivity and recipient resistance is described and appears to have future value as a generalized selective device for use after matings.