Open Accessbla NDM-1 Is a Chimera Likely Constructed in Acinetobacter baumannii
Author(s) -
Mark A. Toleman,
James Spencer,
Lindsey Jones,
Timothy R. Walsh
Publication year - 2012
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.06297-11
Subject(s) - chimera (genetics) , acinetobacter baumannii , gene , biology , acinetobacter , genetics , dna , bacteria , pseudomonas aeruginosa
Alignment of DNA sequences found upstream of aphA6 and all bla(NDM-1) genes displays 100% identity. This identity continues 19 bp into the bla(NDM-1) gene such that the first 6 amino acids of aphA6 and bla(NDM-1) are the same. Furthermore, the percent GC content (GC%) of aphA6 is considerably lower than that of bla(NDM-1) and the GC% within the bla(NDM-1) structural gene changes dramatically after the first 19 bp. This is unequivocal evidence that bla(NDM-1) is a chimera.
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