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The ribosomal L3 protein was identified as a novel target in linezolid (LZD)-resistantMycobacterium tuberculosis strains. Next-generation sequencing confirmedrplC T460C as the sole mutation in an LZD-resistantM. tuberculosis H37Rv strain selectedin vitro . Sequencing analysis revealed therplC T460C mutation in eight further LZD-resistant isolates (threein vitro -selected mutants and five patient isolates, including isolates from three different patients that developed LZD resistance during treatment) but in none of the susceptible control strains (n = 84).

rplC T460C Identified as a Dominant Mutation in Linezolid-Resistant Mycobacterium tuberculosis Strains