Open AccessIMP-29, a Novel IMP-Type Metallo-β-Lactamase in Pseudomonas aeruginosa
Author(s) -
Katy Jeannot,
Laurent Poirel,
Marjorie Robert-Nicoud,
Pascal Cholley,
Patrice Nordmann,
Patrick Plésiat
Publication year - 2012
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.05838-11
Subject(s) - integron , pseudomonas aeruginosa , biology , microbiology and biotechnology , plasmid , strain (injury) , pseudomonadaceae , gene , antibacterial agent , gene cassette , bacteria , genetics , antibiotics , anatomy
Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The bla(IMP-29) gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.
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