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In vitro testing of Aspergillus fumigatus susceptibility to echinocandins has always been a challenge. Using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of β-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors.

antimicrobial agents and chemotherapy

The Composition of the Culture Medium Influences the β-1,3-Glucan Metabolism of Aspergillus fumigatus and the Antifungal Activity of Inhibitors of β-1,3-Glucan Synthesis