Evolution and Single-Nucleotide Polymorphisms in Methicillin-Resistant Staphylococcus aureus Strains with Reduced Susceptibility to Vancomycin and Daptomycin, Based on Determination of the Complete Genome | Zendy

Tetsuo Yamaguchi | Zendy; Shingo Suzuki | Zendy; Sakiko Okamura | Zendy; Yuri Miura | Zendy; Ayaka Tsukimori | Zendy; Itaru Nakamura | Zendy; Norihiko Ito | Zendy; Anri Masuya | Zendy; Takashi Shiina | Zendy; Tetsuya Matsumoto | Zendy

Open Access

Evolution and Single-Nucleotide Polymorphisms in Methicillin-Resistant Staphylococcus aureus Strains with Reduced Susceptibility to Vancomycin and Daptomycin, Based on Determination of the Complete Genome

Author(s) -

Tetsuo Yamaguchi,

Shingo Suzuki,

Sakiko Okamura,

Yuri Miura,

Ayaka Tsukimori,

Itaru Nakamura,

Norihiko Ito,

Anri Masuya,

Takashi Shiina,

Tetsuya Matsumoto

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.05159-14

Subject(s) - daptomycin , rpob , staphylococcus aureus , biology , microbiology and biotechnology , methicillin resistant staphylococcus aureus , single nucleotide polymorphism , vancomycin , staphylococcal infections , genotype , genetics , gene , bacteria , 16s ribosomal rna

We obtained a series of methicillin-resistantStaphylococcus aureus isolates, including both daptomycin-susceptible strain TD1 and daptomycin-resistant strain TD4, from a patient. We determined the complete genome sequences of TD1 and TD4 using next-generation sequencing, and only four single-nucleotide polymorphisms (SNPs) were identified, one each incapB (E58K),rpoB (H481Y),lytN (I16V), andmprF (V351E). We determined that these four SNPs were sufficient to cause the strains to develop daptomycin, vancomycin, and rifampin resistance.

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