Phenotypic and Genotypic Characterization of Pyrazinamide Resistance among Multidrug-Resistant Mycobacterium tuberculosis Isolates in Zhejiang, China | Zendy

Qiang Xia | Zendy; Lili Zhao | Zendy; Feng Li | Zendy; Yumei Fan | Zendy; Yuanyuan Chen | Zendy; Beibei Wu | Zendy; Zhengwei Liu | Zendy; Aizhen Pan | Zendy; Min Zhu | Zendy

Open Access

Phenotypic and Genotypic Characterization of Pyrazinamide Resistance among Multidrug-Resistant Mycobacterium tuberculosis Isolates in Zhejiang, China

Author(s) -

Qiang Xia,

Lili Zhao,

Feng Li,

Yumei Fan,

Yuanyuan Chen,

Beibei Wu,

Zhengwei Liu,

Aizhen Pan,

Min Zhu

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.04541-14

Subject(s) - pyrazinamide , mycobacterium tuberculosis , biology , genotype , drug resistance , multiple drug resistance , microbiology and biotechnology , tuberculosis , streptomycin , genetics , virology , gene , medicine , rifampicin , antibiotics , pathology

To explore the phenotypic and genotypic characterization of pyrazinamide (PZA) resistance among multidrug-resistantMycobacterium tuberculosis (MDR-TB) isolates in Zhejiang province, a total of 274 MDR-TB isolates were collected. Drug susceptibility testing and spoligotyping were performed on all clinical isolates. In addition, the mutated features of PZA-resistant loci, includingpncA andrpsA , were also analyzed by DNA sequencing. Our results showed that the prevalence of PZA resistance among MDR-TB strains in Zhejiang province was 43.07% and that PZA resistance was associated with concomitant resistance to streptomycin. The majority of PZA-resistant MDR-TB isolates belonged to the Beijing family. Mutations withinpncA , notrpsA , constituted the primary mechanism of PZA resistance. Among 118 PZA-resistant isolates, 53 different mutations were observed inpncA , and most of them were point mutations. Compared with the phenotypic data, DNA sequencing ofpncA has sensitivity and specificity of 77.97% and 96.79%, respectively. Analysis ofpncA provided a robust tool for rapid detection of PZA drug resistance.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research