Open AccessEvolution of Staphylococcus aureus under Vancomycin Selective Pressure: the Role of the Small-Colony Variant Phenotype
Author(s) -
Justin R. Lenhard,
Christof von Eiff,
Irene S. Hong,
Patricia N. Holden,
Michael D. Bear,
Amy Suen,
Zackery P. Bulman,
Brian T. Tsuji
Publication year - 2014
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.04508-14
Subject(s) - staphylococcus aureus , vancomycin , microbiology and biotechnology , micrococcaceae , antibiotics , phenotype , population , methicillin resistant staphylococcus aureus , biology , antibacterial agent , bacteria , staphylococcal infections , strain (injury) , medicine , gene , genetics , environmental health , anatomy
Staphylococcus aureus small-colony variants (SCVs) often persist despite antibiotic therapy. Against a 108 -CFU/ml methicillin-resistantS. aureus (MRSA) (strain COL) population of which 0%, 1%, 10%, 50%, or 100% was an isogenichemB knockout (Ia48) subpopulation displaying the SCV phenotype, vancomycin achieved maximal reductions of 4.99, 5.39, 4.50, 3.28, and 1.66 log10 CFU/ml over 48 h. Vancomycin at ≥16 mg/liter shifted a population from 50% SCV cells at 0 h to 100% SCV cells at 48 h, which was well characterized by a Hill-type model (R 2 > 0.90).
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