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For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, thein vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.

antimicrobial agents and chemotherapy

Evaluation of a Modified EUCAST Fragmented-Mycelium Inoculum Method for <i>In Vitro</i> Susceptibility Testing of Dermatophytes and the Activity of Novel Antifungal Agents

Evaluation of a Modified EUCAST Fragmented-Mycelium Inoculum Method for In Vitro Susceptibility Testing of Dermatophytes and the Activity of Novel Antifungal Agents