Evaluation of a Modified EUCAST Fragmented-Mycelium Inoculum Method for In Vitro Susceptibility Testing of Dermatophytes and the Activity of Novel Antifungal Agents | Zendy

Brigitte Risslegger | Zendy; Cornelia LassFlörl | Zendy; Gerhard Blum | Zendy; Michaela Lackner | Zendy

Open Access

Evaluation of a Modified EUCAST Fragmented-Mycelium Inoculum Method for In Vitro Susceptibility Testing of Dermatophytes and the Activity of Novel Antifungal Agents

Author(s) -

Brigitte Risslegger,

Cornelia LassFlörl,

Gerhard Blum,

Michaela Lackner

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.04381-14

Subject(s) - broth microdilution , posaconazole , mycelium , microbiology and biotechnology , antifungal , echinocandins , biology , amphotericin b , minimum inhibitory concentration , antibiotics , botany , caspofungin

For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, thein vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.

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