Molecular and Biochemical Characterization of CTX-M-131, a Natural Asp240Gly Variant Derived from CTX-M-2, Produced by a Providencia rettgeri Clinical Strain in São Paulo, Brazil | Zendy

Milena Dropa | Zendy; Bárbara Ghiglione | Zendy; Maria Helena Matté | Zendy; Lívia Carminato Balsalobre | Zendy; Nilton Lincopán | Zendy; Glavur Rogério Matté | Zendy; Gabriel Gutkind | Zendy; P.P. Power | Zendy

Open Access

Molecular and Biochemical Characterization of CTX-M-131, a Natural Asp240Gly Variant Derived from CTX-M-2, Produced by a Providencia rettgeri Clinical Strain in São Paulo, Brazil

Author(s) -

Milena Dropa,

Bárbara Ghiglione,

Maria Helena Matté,

Lívia Carminato Balsalobre,

Nilton Lincopán,

Glavur Rogério Matté,

Gabriel Gutkind,

P.P. Power

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.04116-14

Subject(s) - providencia , integron , plasmid , strain (injury) , enzyme , enterobacteriaceae , mutation , biology , microbiology and biotechnology , ceftazidime , gene , bacteria , genetics , biochemistry , escherichia coli , pseudomonas aeruginosa , anatomy

CTX-M-131 is a natural Asp240Gly variant from the CTX-M-2 group detected in aProvidencia rettgeri clinical strain from Brazil. Molecular analysis showed thatbla CTX-M-131 was inserted in a complex class 1 integron harbored by a 112-kb plasmid, which has not been previously described as a platform for CTX-M-encoding genes with the Asp240Gly mutation. Steady-state kinetic parameters showed that the enzyme has a typical cefotaximase catalytic profile and an enhanced activity against ceftazidime.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research