Evaluation of a Loop-Mediated Isothermal Amplification-Based Methodology To Detect Carbapenemase Carriage in Acinetobacter Clinical Isolates | Zendy

Andrea Vergara | Zendy; Yuliya Zboromyrska | Zendy; Noraida Mosqueda | Zendy; María Isabel Morosini | Zendy; Sergio GarcíaFernández | Zendy; Ignasi Roca | Zendy; Rafael Cantón | Zendy; Francesc Marco | Zendy; Jordi Vilà | Zendy

Open Access

Evaluation of a Loop-Mediated Isothermal Amplification-Based Methodology To Detect Carbapenemase Carriage in Acinetobacter Clinical Isolates

Author(s) -

Andrea Vergara,

Yuliya Zboromyrska,

Noraida Mosqueda,

María Isabel Morosini,

Sergio GarcíaFernández,

Ignasi Roca,

Rafael Cantón,

Francesc Marco,

Jordi Vilà

Publication year - 2014

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.03870-14

Subject(s) - carriage , acinetobacter , acinetobacter baumannii , loop mediated isothermal amplification , microbiology and biotechnology , carbapenem , biology , neisseriaceae , medicine , bacteria , antibiotics , pseudomonas aeruginosa , genetics , dna , pathology

Carbapenem-resistantAcinetobacter baumannii is a major source of nosocomial infections worldwide and is mainly associated with the acquisition of OXA-type carbapenemases and, to a lesser extent, metallo-β-lactamases (MBLs). In this study, 82 nonepidemiologically relatedAcinetobacter strains carrying different types of OXA or MBL enzymes were tested using the Eazyplex system, a loop-mediated isothermal amplification (LAMP)-based method to rapidly detect carbapenemase carriage. The presence/absence of carbapenem-hydrolyzing enzymes was correctly determined for all isolates in <30 min.

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