Identification of a Novel 6′- N -Aminoglycoside Acetyltransferase, AAC(6′)-Iak, from a Multidrug-Resistant Clinical Isolate of Stenotrophomonas maltophilia | Zendy

Tatsuya Tada | Zendy; Tohru MiyoshiAkiyama | Zendy; Rajan K. Dahal | Zendy; Shyam Kumar Mishra | Zendy; Kayo Shimada | Zendy; Hiroshi Ohara | Zendy; Teruo Kirikae | Zendy; Bharat Mani Pokhrel | Zendy

Open Access

Identification of a Novel 6′- N -Aminoglycoside Acetyltransferase, AAC(6′)-Iak, from a Multidrug-Resistant Clinical Isolate of Stenotrophomonas maltophilia

Author(s) -

Tatsuya Tada,

Tohru MiyoshiAkiyama,

Rajan K. Dahal,

Shyam Kumar Mishra,

Kayo Shimada,

Hiroshi Ohara,

Teruo Kirikae,

Bharat Mani Pokhrel

Publication year - 2014

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.03354-14

Subject(s) - sisomicin , netilmicin , tobramycin , microbiology and biotechnology , aminoglycoside , amikacin , stenotrophomonas maltophilia , gentamicin , chemistry , biology , antibiotics , pseudomonas aeruginosa , bacteria , genetics

Stenotrophomonas maltophilia IOMTU250 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Iak. The encoded protein, AAC(6')-Iak, consists of 153 amino acids and has 86.3% identity to AAC(6')-Iz. Escherichia coli transformed with a plasmid containing aac(6')-Iak exhibited decreased susceptibility to arbekacin, dibekacin, neomycin, netilmicin, sisomicin, and tobramycin. Thin-layer chromatography showed that AAC(6')-Iak acetylated amikacin, arbekacin, dibekacin, isepamicin, kanamycin, neomycin, netilmicin, sisomicin, and tobramycin but not apramycin, gentamicin, or lividomycin.

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