Inactivation of Lytic Transglycosylases Increases Susceptibility to Aminoglycosides and Macrolides by Altering the Outer Membrane Permeability of Stenotrophomonas maltophilia | Zendy

Chao-Jung Wu | Zendy; Yi-Wei Huang | Zendy; Yi-Tsung Lin | Zendy; TsueyChing Yang | Zendy

Open Access

Inactivation of Lytic Transglycosylases Increases Susceptibility to Aminoglycosides and Macrolides by Altering the Outer Membrane Permeability of Stenotrophomonas maltophilia

Author(s) -

Chao-Jung Wu,

Yi-Wei Huang,

Yi-Tsung Lin,

TsueyChing Yang

Publication year - 2016

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.03026-15

Subject(s) - stenotrophomonas maltophilia , lytic cycle , bacterial outer membrane , efflux , microbiology and biotechnology , membrane permeability , chemistry , biology , bacteria , biochemistry , membrane , pseudomonas aeruginosa , escherichia coli , gene , virology , genetics , virus

Stenotrophomonas maltophilia harbors six lytic transglycosylases (LTs):mltA ,mltB1 ,mltB2 ,mltD1 ,mltD2 , andslt . LT deletion increased susceptibility ofS. maltophilia to aminoglycosides (AGs) and macrolides, and the underlying mechanisms were investigated. The expression of AG-modifying enzymes and efflux pumps was evaluated by quantitative reverse transcription-PCR (qRT-PCR). Susceptibility to 1-N -phenylnaphthylamine, vancomycin, SDS, and bile salts was measured to assess outer membrane permeability. In conclusion, increased outer membrane permeability contributes to LT deletion-mediated increase in aminoglycoside and macrolide susceptibility.

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