Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility | Zendy

Catiana Dudiuk | Zendy; Daiana Macedo | Zendy; Florencia Leonardelli | Zendy; Laura Theill | Zendy; Matías S. Cabeza | Zendy; Soledad Gamarra | Zendy; Guillermo GarcíaEffrón | Zendy

Open Access

Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility

Author(s) -

Catiana Dudiuk,

Daiana Macedo,

Florencia Leonardelli,

Laura Theill,

Matías S. Cabeza,

Soledad Gamarra,

Guillermo GarcíaEffrón

Publication year - 2017

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02644-16

Subject(s) - echinocandin , mutant , biology , saccharomyces cerevisiae , gene , mutation , microbiology and biotechnology , fungal protein , genetics , antifungal , fluconazole

Candida guilliermondii shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) in the Fks1p hot spot 1 region. Our objective was to confirm that the reduced echinocandin susceptibility ofC. guilliermondii is due to those naturally occurring substitutions. We constructed aSaccharomyces cerevisiae mutant in which a region of theFKS1 gene (including hot spot 1) was replaced with that fromC. guilliermondii . The chimeric mutants showed 32-fold increases in echinocandin MIC values, confirming the hypothesis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research