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Complete nucleotide sequences were determined for two plasmids bearingrmtD group 16S rRNA methyltransferase genes. pKp64/11 was 78 kb in size, belonged to the IncL/M group, and harboredbla TEM-1b ,sul1 ,qacE Δ1 ,dfrA22 , andrmtD1 across two multidrug resistance regions (MRRs). pKp368/10 was 170 kb in size, belonged to the IncA/C group, and harboredacrB ,sul1 ,qacE Δ1 ,ant(3″)-Ia ,aac(6′)-Ib ,cat ,rmtD2 , andbla CTX-M-8 across three MRRs. ThermtD -containing regions shared a conserved motif, suggesting a common origin for the twormtD alleles.

Complete Sequences of Multidrug Resistance Plasmids Bearing rmtD1 and rmtD2 16S rRNA Methyltransferase Genes