Patient-Derived Cytomegaloviruses with Different Ganciclovir Sensitivities from UL97 Mutation Retain Their Replication Efficiency and Some Kinase Activity In Vitro | Zendy

Diana Wong | Zendy; Wendy J. van Zuylen | Zendy; Stuart T. Hamilton | Zendy; Mirjam Steingruber | Zendy; Eric Sonntag | Zendy; Manfred Marschall | Zendy; William D﻿.﻿ Rawlinson | Zendy

Open Access

Patient-Derived Cytomegaloviruses with Different Ganciclovir Sensitivities from UL97 Mutation Retain Their Replication Efficiency and Some Kinase Activity In Vitro

Author(s) -

Diana Wong,

Wendy J. van Zuylen,

Stuart T. Hamilton,

Mirjam Steingruber,

Eric Sonntag,

Manfred Marschall,

William D. Rawlinson

Publication year - 2019

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02425-18

Subject(s) - ganciclovir , in vitro , mutation , biology , virology , replication (statistics) , genetics , kinase , microbiology and biotechnology , gene , virus , human cytomegalovirus

Mutations in the cytomegalovirus UL97 kinase gene contribute to antiviral resistance. Mutations A594S and G598D from two clinical isolates were analyzed, and bacterial artificial chromosome (BAC)-engineered A594S recombinant cytomegalovirus exhibited a ganciclovir-resistant phenotype on plaque reduction. Viral replication was comparable to that of the wild type. Cell-based kinase activity and autophosphorylation of ectopically expressed proteins showed that mutants retained some kinase activity.

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