Open AccessMolecular Epidemiology of NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii Isolates from Pakistan
Author(s) -
Anna L. Sartor,
Muhammad W. Raza,
Shahid Ahmed Abbasi,
Kathryn M. Day,
John D. Perry,
David L. Paterson,
Hanna E. Sidjabat
Publication year - 2014
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02425-14
Subject(s) - biology , enterobacteriaceae , acinetobacter baumannii , replicon , enterobacter cloacae , molecular epidemiology , plasmid , microbiology and biotechnology , multilocus sequence typing , typing , acinetobacter , enterobacter , genetics , escherichia coli , gene , antibiotics , bacteria , genotype , pseudomonas aeruginosa
The molecular epidemiology of 66 NDM-producing isolates from 2 Pakistani hospitals was investigated, with their genetic relatedness determined using repetitive sequence-based PCR (Rep-PCR). PCR-based replicon typing and screening for antibiotic resistance genes encoding carbapenemases, other β-lactamases, and 16S methylases were also performed. Rep-PCR suggested a clonal spread of Enterobacter cloacae and Escherichia coli. A number of plasmid replicon types were identified, with the incompatibility A/C group (IncA/C) being the most common (78%). 16S methylase-encoding genes were coharbored in 81% of NDM-producing Enterobacteriaceae.
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