Identification of CMY-2-Type Cephalosporinases in Clinical Isolates of Enterobacteriaceae by MALDI-TOF MS | Zendy

Costas C. Papagiannitsis | Zendy; Stathis D. Kotsakis | Zendy; Zdeněk Tůma | Zendy; Marek Gniadkowski | Zendy; Vivì Miriagou | Zendy; Jaroslav Hrabák | Zendy

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Identification of CMY-2-Type Cephalosporinases in Clinical Isolates of Enterobacteriaceae by MALDI-TOF MS

Author(s) -

Costas C. Papagiannitsis,

Stathis D. Kotsakis,

Zdeněk Tůma,

Marek Gniadkowski,

Vivì Miriagou,

Jaroslav Hrabák

Publication year - 2014

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02418-13

Subject(s) - citrobacter freundii , matrix assisted laser desorption/ionization , enterobacteriaceae , microbiology and biotechnology , mass spectrometry , chemistry , periplasmic space , chromatography , biology , escherichia coli , biochemistry , desorption , gene , organic chemistry , adsorption

This study exploited the possibility to detect Citrobacter freundii-derived CMY-2-like cephalosporinases in Enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Periplasmic proteins were prepared using a modified sucrose method and analyzed by MALDI-TOF MS. A ca. 39,850-m/z peak, confirmed to represent a C. freundii-like β-lactamase by in-gel tryptic digestion followed by MALDI-TOF/TOF MS, was observed only in CMY-producing isolates. We have also shown the potential of the assay to detect ACC- and DHA-like AmpC-type β-lactamases.

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