In Vitro Antifungal Susceptibility Testing of Candida Isolates with the EUCAST Methodology, a New Method for ECOFF Determination | Zendy

Joseph Meletiadis | Zendy; Ilse Curfs-Breuker | Zendy; Jacques F. Meis | Zendy; Johan W. Mouton | Zendy

Open Access

In Vitro Antifungal Susceptibility Testing of Candida Isolates with the EUCAST Methodology, a New Method for ECOFF Determination

Author(s) -

Joseph Meletiadis,

Ilse Curfs-Breuker,

Jacques F. Meis,

Johan W. Mouton

Publication year - 2017

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02372-16

Subject(s) - broth microdilution , candida albicans , echinocandins , microbiology and biotechnology , biology , corpus albicans , candida krusei , minimum inhibitory concentration , antifungal , amphotericin b , antibiotics , caspofungin

Thein vitro susceptibilities of 1,099 molecularly identified clinicalCandida isolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs. Overall, low non-wild-type/resistance rates were found. The highest rates were found for azoles withC. parapsilosis (2.7 to 9.8%),C. albicans (7%), andC. glabrata (1.7 to 2.3%) and for echinocandins withC. krusei (3.3%),C. albicans (1%), andC. tropicalis (1.7%).

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