Open AccessNovel Polyanions Inhibiting Replication of Influenza Viruses
Author(s) -
Justyna Ciejka,
Aleksandra Milewska,
Magdalena Wytrwał-Sarna,
Jacek Wojarski,
Anna Gołda,
Marek Ochman,
Maria Nowakowska,
Krzysztof Szczubiałka,
Krzysztof Pyrć
Publication year - 2016
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02183-15
Subject(s) - polysaccharide , chemistry , allylamine , virus , microbiology and biotechnology , cell culture , molecular mass , influenza a virus , hydrochloride , biology , biochemistry , virology , polyelectrolyte , genetics , organic chemistry , polymer , enzyme
Novel sulfonated derivatives of poly(allylamine hydrochloride) (NSPAHs) and N-sulfonated chitosan (NSCH) have been synthesized, and their activity against influenza A and B viruses has been studied and compared with that of a series of carrageenans, marine polysaccharides of well-documented anti-influenza activity. NSPAHs were found to be nontoxic and very soluble in water, in contrast to gel-forming and thus generally poorly soluble carrageenans.In vitro andex vivo studies using susceptible cells (Madin-Darby canine kidney epithelial cells and fully differentiated human airway epithelial cultures) demonstrated the antiviral effectiveness of NSPAHs. The activity of NSPAHs was proportional to the molecular mass of the chain and the degree of substitution of amino groups with sulfonate groups. Mechanistic studies showed that the NSPAHs and carrageenans inhibit influenza A and B virus assembly in the cell.
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