Function of Cytochrome P450 Enzymes RosC and RosD in the Biosynthesis of Rosamicin Macrolide Antibiotic Produced by Micromonospora rosaria | Zendy

Yohei Iizaka | Zendy; Noriko Higashi | Zendy; Masanari Ishida | Zendy; Reina Oiwa | Zendy; Yumi Ichikawa | Zendy; Moeka Takeda | Zendy; Yojiro Anzai | Zendy; Fumio Kato | Zendy

Open Access

Function of Cytochrome P450 Enzymes RosC and RosD in the Biosynthesis of Rosamicin Macrolide Antibiotic Produced by Micromonospora rosaria

Author(s) -

Yohei Iizaka,

Noriko Higashi,

Masanari Ishida,

Reina Oiwa,

Yumi Ichikawa,

Moeka Takeda,

Yojiro Anzai,

Fumio Kato

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02092-12

Subject(s) - micromonospora , complementation , cytochrome p450 , biology , escherichia coli , enzyme , gene , gene cluster , biosynthesis , biochemistry , genetics , bacteria , streptomyces , phenotype

The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.

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