attI1 -Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence | Zendy

Costas C. Papagiannitsis | Zendy; L. S. Tzouvelekis | Zendy; Εva Tzelepi | Zendy; Vivì Miriagou | Zendy

Open Access

attI1 -Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence

Author(s) -

Costas C. Papagiannitsis,

L. S. Tzouvelekis,

Εva Tzelepi,

Vivì Miriagou

Publication year - 2016

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02070-16

Subject(s) - open reading frame , integron , genetics , genbank , gene , upstream open reading frame , biology , reading (process) , plasmid , reading frame , frame (networking) , linguistics , peptide sequence , computer science , philosophy , telecommunications

By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within theattI1 site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (bla GES-1 ) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression.

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