Contribution of β-Lactamases and Porin Proteins OmpK35 and OmpK36 to Carbapenem Resistance in Clinical Isolates of KPC-2-Producing Klebsiella pneumoniae | Zendy

Ying Zhang | Zendy; Xiaofei Jiang | Zendy; Yanyan Wang | Zendy; Gang Li | Zendy; Yueru Tian | Zendy; Hong Liu | Zendy; Fuqi Ai | Zendy; Yiming Ma | Zendy; Bei Wang | Zendy; Fei-yi Ruan | Zendy; Kumar Rajakumar | Zendy

Open Access

Contribution of β-Lactamases and Porin Proteins OmpK35 and OmpK36 to Carbapenem Resistance in Clinical Isolates of KPC-2-Producing Klebsiella pneumoniae

Author(s) -

Ying Zhang,

Xiaofei Jiang,

Yanyan Wang,

Gang Li,

Yueru Tian,

Hong Liu,

Fuqi Ai,

Yiming Ma,

Bei Wang,

Fei-yi Ruan,

Kumar Rajakumar

Publication year - 2013

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02045-12

Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , porin , biology , carbapenem , enterobacteriaceae , klebsiella , escherichia coli , gene , genetics , antibiotics , bacterial outer membrane

Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.

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