Identification of the Novel Lincosamide Resistance Gene lnu (E) Truncated by ISEnfa5-cfr-IS Enfa5 Insertion in Streptococcus suis: De Novo Synthesis and Confirmation of Functional Activity in Staphylococcus aureus | Zendy

Qin Zhao | Zendy; Sarah Wendlandt | Zendy; Hui Li | Zendy; Jun Li | Zendy; Congming Wu | Zendy; Jianzhong Shen | Zendy; Štefan Schwarz | Zendy; Yang Wang | Zendy

Open Access

Identification of the Novel Lincosamide Resistance Gene lnu (E) Truncated by ISEnfa5-cfr-IS Enfa5 Insertion in Streptococcus suis: De Novo Synthesis and Confirmation of Functional Activity in Staphylococcus aureus

Author(s) -

Qin Zhao,

Sarah Wendlandt,

Hui Li,

Jun Li,

Congming Wu,

Jianzhong Shen,

Štefan Schwarz,

Yang Wang

Publication year - 2013

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.02007-13

Subject(s) - lincomycin , biology , streptococcus suis , shuttle vector , gene , lincosamides , microbiology and biotechnology , genetics , staphylococcus aureus , bacteria , vector (molecular biology) , antibiotics , recombinant dna , virulence

The novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with ≤69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.

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