SmeOP-TolC Sm Efflux Pump Contributes to the Multidrug Resistance of Stenotrophomonas maltophilia | Zendy

ChengWen Lin | Zendy; Yi-Wei Huang | Zendy; Rouh-Mei Hu | Zendy; TsueyChing Yang | Zendy

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SmeOP-TolC _Sm Efflux Pump Contributes to the Multidrug Resistance of Stenotrophomonas maltophilia

Author(s) -

ChengWen Lin,

Yi-Wei Huang,

Rouh-Mei Hu,

TsueyChing Yang

Publication year - 2014

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01974-13

Subject(s) - stenotrophomonas maltophilia , efflux , microbiology and biotechnology , multiple drug resistance , tetr , fosfomycin , biology , chemistry , repressor , biochemistry , drug resistance , antibiotics , gene , pseudomonas aeruginosa , bacteria , gene expression , genetics

A five-gene cluster,tolCSm -pcm-smeRo-smeO-smeP, ofStenotrophomonas maltophilia was characterized. The presence ofsmeOP andsmeRo-pcm-tolCSm operons was verified by reverse transcription (RT)-PCR. Both operons were negatively regulated by the TetR-type transcriptional regulator SmeRo, as demonstrated by quantitative RT-PCR and a promoter-fusion assay. SmeO and SmeP were associated with TolCSm (the TolC protein ofS. maltophilia ) for the assembly of a resistance-nodulation-cell-division (RND)-type pump. The compounds extruded by SmeOP-TolCSm mainly included nalidixic acid, doxycycline, amikacin, gentamicin, erythromycin, leucomycin, carbonyl cyanide 3-chlorophenylhydrazone, crystal violet, sodium dodecyl sulfate, and tetrachlorosalicylanilide.

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