Open AccessIdentification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis
Author(s) -
Michael Sonnabend,
Kristina Klein,
Sina Beier,
Angel Angelov,
Robert Maria Kluj,
Christoph Mayer,
Caspar Groß,
Kathrin Hofmeister,
Antonia Beuttner,
Matthias Willmann,
Silke Peter,
Philipp Oberhettinger,
Annika Schmidt,
Ingo B. Autenrieth,
Monika Schütz,
Erwin Bohn
Publication year - 2020
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01771-19
Subject(s) - resistome , biology , microbiology and biotechnology , pseudomonas aeruginosa , cefepime , meropenem , transposon mutagenesis , antibiotics , mutant , cefpirome , transposable element , drug resistance , antibiotic resistance , gene , imipenem , genetics , bacteria , integron
With the aim to identify potential new targets to restore antimicrobial susceptibility of multidrug-resistant (MDR) Pseudomonas aeruginosa isolates, we generated a high-density transposon (Tn) insertion mutant library in an MDR P. aeruginosa bloodstream isolate (isolate ID40). The depletion of Tn insertion mutants upon exposure to cefepime or meropenem was measured in order to determine the common resistome for these clinically important antipseudomonal β-lactam antibiotics.