Open AccessSymmetry Requirements for Effective Blocking of Pore-Forming Toxins: Comparative Study with α-, β-, and γ-Cyclodextrin Derivatives
Author(s) -
Konstantina Yannakopoulou,
László Jicsinszky,
Crysie Aggelidou,
Nikolaos Mourtzis,
Tanisha M. Robinson,
Adiamseged Yohannes,
Ekaterina M. Nestorovich,
Sergey M. Bezrukov,
Vladimir A. Karginov
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01764-10
Subject(s) - bacillus anthracis , anthrax toxin , cyclodextrin , toxin , hemolysin , chemistry , blocking (statistics) , cationic polymerization , staphylococcus aureus , protein subunit , biophysics , biochemistry , biology , bacteria , organic chemistry , virulence , genetics , statistics , mathematics , gene , fusion protein , recombinant dna
We compared the abilities of structurally related cationic cyclodextrins to inhibitBacillus anthracis lethal toxin andStaphylococcus aureus α-hemolysin. We found that both β- and γ-cyclodextrin derivatives effectively inhibited anthrax toxin action by blocking the transmembrane oligomeric pores formed by the protective antigen (PA) subunit of the toxin, whereas α-cyclodextrins were ineffective. In contrast, α-hemolysin was selectively blocked only by β-cyclodextrin derivatives, demonstrating that both symmetry and size of the inhibitor and the pore are important.