Open AccessEvolution and Spread of a Multidrug-Resistant Proteus mirabilis Clone with Chromosomal AmpC-Type Cephalosporinases in Europe
Author(s) -
Marco Maria D’Andrea,
Elżbieta Literacka,
A. Zioga,
Tommaso Giani,
Anna Baraniak,
Janusz Fiett,
Ewa Sadowy,
Panayotis T. Tassios,
Gian María Rossolini,
Marek Gniadkowski,
Vivì Miriagou
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01736-10
Subject(s) - proteus mirabilis , multiple drug resistance , clone (java method) , biology , microbiology and biotechnology , genetics , gene , drug resistance , escherichia coli
Proteus mirabilis isolates obtained in 1999 to 2008 from three European countries were analyzed; all carried chromosomal AmpC-type cephalosporinasebla CMY genes from aCitrobacter freundii origin (bla CMY-2 -like genes). Isolates from Poland harbored severalbla CMY genes (bla CMY-4 ,bla CMY-12 ,bla CMY-14 ,bla CMY-15 , andbla CMY-38 and the new genebla CMY-45 ), while isolates from Italy and Greece harboredbla CMY-16 only. Earlier isolates withbla CMY-4 orbla CMY-12 , recovered in France from Greek and Algerian patients, were also studied. All isolates showed striking similarities. Theirbla CMY genes resided within ISEcp1 transposition modules, named Tn6093 , characterized by a 110-bp distance between ISEcp1 andbla CMY , and identical fragments of bothC. freundii DNA and a ColE1-type plasmid backbone. Moreover, these modules were inserted into the same chromosomal site, within thepepQ gene. Since ColE1 plasmids carrying ISEcp1 with similarC. freundii DNA fragments (Tn6114 ) had been identified earlier, it is likely that a similar molecule had mediated at some stage this DNA transfer betweenC. freundii andP. mirabilis . In addition, isolates withbla CMY-12 ,bla CMY-15 , andbla CMY-38 genes harbored a secondbla CMY copy within a shorter ISEcp1 module (Tn6113 ), always inserted downstream of theppiD gene. Sequence analysis of all mobilebla CMY-2 -like genes indicated that those integrated in theP. mirabilis chromosome form a distinct cluster that may have evolved by the stepwise accumulation of mutations. All of these observations, coupled to strain typing data, suggest that thebla CMY genes studied here may have originated from a single ISEcp1 -mediated mobilization-transfer-integration process, followed by the spread and evolution of aP. mirabilis clone over time and a large geographic area.