Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides | Zendy

Shaohua Zhao | Zendy; Sampa Mukherjee | Zendy; Cong Li | Zendy; Sonya Bodeis Jones | Zendy; Shenia Young | Zendy; Patrick F. McDermott | Zendy

Open Access

Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides

Author(s) -

Shaohua Zhao,

Sampa Mukherjee,

Cong Li,

Sonya Bodeis Jones,

Shenia Young,

Patrick F. McDermott

Publication year - 2017

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01682-17

Subject(s) - kanamycin , biology , aminoglycoside , amikacin , microbiology and biotechnology , gentamicin , tobramycin , campylobacter , gene , cloning (programming) , genetics , antibiotics , bacteria , computer science , programming language

Nineaph genes, includingaph(2″)-Ib ,aph(2″)-Ic ,aph(2″)-Ig ,aph(2″)-If ,aph(2″)-If1 ,aph(2″)-If3 ,aph(2″)-Ih ,aac(6′)-Ie –aph(2″)-Ia , andaac(6′)-Ie –aph(2″)-If2 , were previously identified inCampylobacter . To measure the contribution of these alleles to aminoglycoside resistance, we cloned nine genes into the pBluescript and expressed them inEscherichia coli DH5α. The nineaph expressed inE. coli showed various levels of resistance to gentamicin, kanamycin, and tobramycin. Three genes,aac(6″)-Ie –aph(2″)-Ia ,aph2″-If1 , andaph2″-Ig , showed increased MICs to amikacin, and fiveaph genes were transferrable.

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