Open AccessMolecular Characterization of β-Lactamase Genes and Their Genetic Structures inAcinetobacterGenospecies 3 Isolates in Taiwan
Author(s) -
Li-Yueh Huang,
PoLiang Lu,
TeLi Chen,
Fang-Yee Chang,
Chang-Phoné Fung,
L. Kristopher Siu
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01624-09
Subject(s) - integron , acinetobacter , biology , gene , integrase , microbiology and biotechnology , genetics , sequence analysis , plasmid , antibiotics
The genetic structure of beta-lactamases in Acinetobacter genospecies 3 (AG3) isolates in Taiwan was studied to analyze their high rates of resistance to beta-lactams, including carbapenems (57.9%). bla(IMP-1) and bla(IMP-8) were located in a class 1 integron. bla(OXA-58) was bracketed by ISAba3. A novel TnpF-like integrase gene was identified upstream of bla(VEB-3). Adjacent to the 5' sequence of the bla(ADC) gene, folE was identified. Four new Acinetobacter-derived cephalosporinase (ADC) enzymes were found, which clustered phylogenetically with published AG3 ADC proteins.