Detection of Carbapenemase-Producing Bacteria by Using an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method | Zendy

Anne Carricajo | Zendy; Paul O. Verhoeven | Zendy; Salim Guezzou | Zendy; Nathalie Fonsale | Zendy; G. Aubert | Zendy

Open Access

Detection of Carbapenemase-Producing Bacteria by Using an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method

Author(s) -

Anne Carricajo,

Paul O. Verhoeven,

Salim Guezzou,

Nathalie Fonsale,

G. Aubert

Publication year - 2013

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01540-13

Subject(s) - acinetobacter baumannii , chromatography , pseudomonas aeruginosa , mass spectrometry , tandem mass spectrometry , bacteria , microbiology and biotechnology , high performance liquid chromatography , liquid chromatography–mass spectrometry , chemistry , biology , genetics

The emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. In a panel of 78 isolates, including 41 carbapenemase-producing strains, the ULPC-MS/MS assay showed 100% agreement with molecular characterization, whereas six carbapenemase-producing isolates were not detected by the modified Hodge test.

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