Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs | Zendy

Kalyan D. Chavda | Zendy; Michael J. Satlin | Zendy; Liang Chen | Zendy; Claudia Manca | Zendy; Stephen G. Jenkins | Zendy; Thomas J. Walsh | Zendy; Barry N. Kreiswirth | Zendy

Open Access

Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs

Author(s) -

Kalyan D. Chavda,

Michael J. Satlin,

Liang Chen,

Claudia Manca,

Stephen G. Jenkins,

Thomas J. Walsh,

Barry N. Kreiswirth

Publication year - 2016

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01458-16

Subject(s) - enterobacteriaceae , chromogenic , microbiology and biotechnology , biology , multiplex , multiplex polymerase chain reaction , agar , plasmid , polymerase chain reaction , virology , bacteria , escherichia coli , gene , genetics , chemistry , chromatography

We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producingEnterobacteriaceae compared to culture on chromogenic agar.

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