Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying the bla NMC-A Carbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex | Zendy

Alberto Antonelli | Zendy; Marco Maria D’Andrea | Zendy; Vincenzo Di Pilato | Zendy; Bruno Viaggi | Zendy; Francesca Torricelli | Zendy; Gian María Rossolini | Zendy

Open Access

Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying the bla _NMC-A Carbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex

Author(s) -

Alberto Antonelli,

Marco Maria D’Andrea,

Vincenzo Di Pilato,

Bruno Viaggi,

Francesca Torricelli,

Gian María Rossolini

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01452-15

Subject(s) - enterobacter cloacae , mobile genetic elements , biology , enterobacter , genome , gene , enterobacteriaceae , chromosome , genetics , microbiology and biotechnology , circular bacterial chromosome , whole genome sequencing , strain (injury) , insertion sequence , dna sequencing , computational biology , escherichia coli , transposable element , anatomy

AnEnterobacter ludwigii strain was isolated during routine screening of a Japanese patient for carriage of carbapenem-resistantEnterobacteriaceae . PCR analysis revealed thebla NMC-A carbapenemase gene. Whole-genome sequencing revealed thatbla NMC-A was inserted in the chromosome and associated with a novel 29.1-kb putative Xer-dependent integrative mobile element, named EludIMEX-1. Bioinformatic analysis identified similar elements in the genomes of anEnterobacter asburiae strain and of otherEnterobacter cloacae complex strains, confirming the mobile nature of this element.

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