Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families | Zendy

Gabriela Bergiante Kraychete | Zendy; Larissa Alvarenga Batista Botelho | Zendy; Eloiza Helena Campana | Zendy; Renata Cristina Picão | Zendy; Raquel Regina Bonelli | Zendy

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Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families

Author(s) -

Gabriela Bergiante Kraychete,

Larissa Alvarenga Batista Botelho,

Eloiza Helena Campana,

Renata Cristina Picão,

Raquel Regina Bonelli

Publication year - 2016

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01447-16

Subject(s) - plasmid , biology , multiplex polymerase chain reaction , gene , genetics , multiplex , computational biology , polymerase chain reaction

Plasmid-mediatedqnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all sixqnr families (includingqnrA ,qnrS ,qnrB ,qnrC ,qnrD , andqnrVC ) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the latest variants described.

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