High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae | Zendy

Aurélie Jayol | Zendy; Patrice Nordmann | Zendy; Adrian Brink | Zendy; María Virginia Villegas | Zendy; Véronique Dubois | Zendy; Laurent Poirel | Zendy

Open Access

High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae

Author(s) -

Aurélie Jayol,

Patrice Nordmann,

Adrian Brink,

María Virginia Villegas,

Véronique Dubois,

Laurent Poirel

Publication year - 2017

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01423-17

Subject(s) - klebsiella pneumoniae , colistin , microbiology and biotechnology , biology , gene , klebsiella infections , klebsiella , enterobacteriaceae , mcr 1 , genetics , escherichia coli , antibiotics

Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.

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