Open AccessCharacterization of Cefoxitin-Resistant Escherichia coli Isolates from Recreational Beaches and Private Drinking Water in Canada between 2004 and 2006
Author(s) -
Laura Mataseje,
Norman F. Neumann,
B. Crago,
Patricia J. Baudry,
George G. Zhanel,
Marie Louie,
Michael R. Mulvey
Publication year - 2009
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01353-08
Subject(s) - cefoxitin , escherichia coli , microbiology and biotechnology , plasmid , biology , multiple drug resistance , replicon , phylogenetic tree , enterobacteriaceae , gene , bacteria , drug resistance , genetics , staphylococcus aureus
A total of 142 cefoxitin-resistantEscherichia coli isolates from water sources were collected across Canada. Multidrug resistance was observed in 65/142 (45.8%) isolates. Thebla CMY-2 gene was identified in 110/142 (77.5%) isolates. Sequencing of the chromosomalampC promoter region showed mutations from the wild type, previously shown to hyperproduce AmpC. CMY-2-producing plasmids predominantly belonged to replicon groups I1-Iγ, A/C, and K/B. The majority of theE. coli isolates belonged to the nonvirulent phylogenetic groups A and B1.