Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates | Zendy

Lisa K. Wong | Zendy; Peera Hemarajata | Zendy; Olusegun O. Soge | Zendy; Romney M. Humphries | Zendy; Jeffrey D. Klausner | Zendy

Open Access

Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates

Author(s) -

Lisa K. Wong,

Peera Hemarajata,

Olusegun O. Soge,

Romney M. Humphries,

Jeffrey D. Klausner

Publication year - 2017

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01339-17

Subject(s) - neisseria gonorrhoeae , cephalosporin , ciprofloxacin , microbiology and biotechnology , virology , biology , minimum inhibitory concentration , antibiotics

Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predictN. gonorrhoeae susceptibility to ESCs by targetingpenA mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predicting at least one ESC MIC above the CDC alert value among clinical isolates, and it could be multiplexed with a previously validatedgyrA PCR to predict ciprofloxacin susceptibility.

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