Genotypic and Phenotypic Evaluation of the Evolution of High-Level Daptomycin Nonsusceptibility in Vancomycin-Resistant Enterococcus faecium | Zendy

Romney M. Humphries | Zendy; Theodoros Kelesidis | Zendy; Ryan Tewhey | Zendy; Warren E. Rose | Zendy; Nicholas J. Schork | Zendy; Victor Nizet | Zendy; George Sakoulas | Zendy

Open Access

Genotypic and Phenotypic Evaluation of the Evolution of High-Level Daptomycin Nonsusceptibility in Vancomycin-Resistant Enterococcus faecium

Author(s) -

Romney M. Humphries,

Theodoros Kelesidis,

Ryan Tewhey,

Warren E. Rose,

Nicholas J. Schork,

Victor Nizet,

George Sakoulas

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01318-12

Subject(s) - daptomycin , enterococcus faecium , enterococcus , genotype , vancomycin , microbiology and biotechnology , biology , vancomycin resistant enterococcus , drug resistance , antibiotics , bacteria , genetics , gene , staphylococcus aureus

Whole-genome sequencing and cell membrane studies of three clonal Enterococcus faecium strains with daptomycin MICs of 4, 32, and 192 μg/ml were performed, revealing nonsynonymous single nucleotide variants in eight open reading frames, including those predicted to encode a phosphoenolpyruvate-dependent, mannose-specific phosphotransferase system, cardiolipin synthetase, and EzrA. Membrane studies revealed a higher net surface charge among the daptomycin-nonsusceptible isolates and increased septum formation in the isolate with a daptomycin MIC of 192 μg/ml.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research