Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages | Zendy

Jérôme Larché | Zendy; Flavie Pouillot | Zendy; Christiane Essoh | Zendy; Balázs Libisch | Zendy; Monica Străuţ | Zendy; Je Chul Lee | Zendy; C. Soler | Zendy; Richard Lamarca | Zendy; Elodie Gleize | Zendy; Jérôme Gabard | Zendy; Gilles Vergnaud | Zendy; Christine Pourcel | Zendy

Open Access

Rapid Identification of International Multidrug-Resistant Pseudomonas aeruginosa Clones by Multiple-Locus Variable Number of Tandem Repeats Analysis and Investigation of Their Susceptibility to Lytic Bacteriophages

Author(s) -

Jérôme Larché,

Flavie Pouillot,

Christiane Essoh,

Balázs Libisch,

Monica Străuţ,

Je Chul Lee,

C. Soler,

Richard Lamarca,

Elodie Gleize,

Jérôme Gabard,

Gilles Vergnaud,

Christine Pourcel

Publication year - 2012

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01233-12

Subject(s) - biology , pseudomonas aeruginosa , lytic cycle , microbiology and biotechnology , multiple drug resistance , genotyping , genotype , virology , multilocus sequence typing , phage typing , locus (genetics) , variable number tandem repeat , typing , tandem repeat , antibiotics , multiple loci vntr analysis , genetics , bacteria , gene , genome , virus

The objective of this study was to determine the genetic diversity of multidrug-resistant (MDR) Pseudomonas aeruginosa strains isolated over a period of 12 months in two French hospitals and to test their susceptibility to bacteriophages. A total of 47 MDR isolates recovered from hospitalized patients were genotyped using multiple-locus variable number of tandem repeats analysis. The genotypes were distributed into five clones (including 19, 5, 5, 3, and 3 isolates, respectively) and 12 singletons. Comparison to 77 MDR strains from three other countries, and MLST analysis of selected isolates showed the predominance of international MDR clones. The larger clone, CC235, contained 59 isolates displaying different antibiotic resistance mechanisms, including the presence of the GES1, VIM-2, VIM-4, and IMP-1 β-lactamases. Three newly isolated P. aeruginosa bacteriophages were found to lyse 42 of the 44 analyzed strains, distributed into the different clonal complexes. This pilot study suggests that systematic genotyping of P. aeruginosa MDR strains could improve our epidemiological understanding of transmission at both the local (hospital) and the national level and that phage therapy could be an alternative or a complementary treatment to antibiotics for treating MDR-infected patients.

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