Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays | Zendy

Omaira Vera | Zendy; Paula Brelas de Brito | Zendy; Letusa Albrecht | Zendy; Keillen Monick Martins-Campos | Zendy; Paulo Filemon Paolucci Pimenta | Zendy; Wuelton Marcelo Monteiro | Zendy; Marcus Lacerda | Zendy; Stefanie Costa Pinto Lopes | Zendy; Fábio Trindade Maranhão Costa | Zendy

Open Access

Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays

Author(s) -

Omaira Vera,

Paula Brelas de Brito,

Letusa Albrecht,

Keillen Monick Martins-Campos,

Paulo Filemon Paolucci Pimenta,

Wuelton Marcelo Monteiro,

Marcus Lacerda,

Stefanie Costa Pinto Lopes,

Fábio Trindade Maranhão Costa

Publication year - 2015

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01136-15

Subject(s) - gametocyte , plasmodium vivax , malaria , virology , plasmodium falciparum , biology , transmission (telecommunications) , plasmodium (life cycle) , blocking (statistics) , impossibility , vivax malaria , parasite hosting , immunology , computer science , telecommunications , computer network , world wide web , political science , law

Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies.

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