Open AccessGenomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China
Author(s) -
Hua Zhou,
Tongwu Zhang,
Dongliang Yu,
Borui Pi,
Qing Yang,
Jianying Zhang,
Songnian Hu,
Yunsong Yu
Publication year - 2011
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01134-10
Subject(s) - acinetobacter baumannii , biology , genetics , multiple drug resistance , transposable element , gene , plasmid , whole genome sequencing , microbiology and biotechnology , insertion sequence , genome , strain (injury) , drug resistance , bacteria , pseudomonas aeruginosa , anatomy
We previously reported that the multidrug-resistant (MDR)Acinetobacter baumannii strain MDR-ZJ06, belonging to European clone II, was widely spread in China. In this study, we report the whole-genome sequence of this clinically important strain. A 38.6-kb AbaR-type genomic resistance island (AbaR22) was identified in MDR-ZJ06. AbaR22 has a structure similar to those of the resistance islands found inA. baumannii strains AYE and AB0057, but it contained only a few antibiotic resistance genes. The region of resistant gene accumulation as previously described was not found in AbaR22. In the chromosome of the strain MDR-ZJ06, we identified the genebla oxa-23 in a composite transposon (Tn2009 ). Tn2009 shared the backbone with otherA. baumannii transponsons that harborbla oxa-23 , but it was bracketed by two ISAba1 elements which were transcribed in the same orientation. MDR-ZJ06 also expressed thearmA gene on its plasmid pZJ06, and this gene has the same genetic environment as thearmA gene of theEnterobacteriaceae . These results suggest variability of resistance acquisition even in closely relatedA. baumannii strains.