Quinolone Resistance in Neisseria gonorrhoeae : Rapid Genotyping of Quinolone Resistance-Determining Regions in gyrA and parC Genes by Melting Curve Analysis Predicts Susceptibility | Zendy

Frédérique Vernel-Pauillac | Zendy; Tiffany R Hogan | Zendy; John W. Tapsall | Zendy; Cyrille Goarant | Zendy

Open Access

Quinolone Resistance in Neisseria gonorrhoeae : Rapid Genotyping of Quinolone Resistance-Determining Regions in gyrA and parC Genes by Melting Curve Analysis Predicts Susceptibility

Author(s) -

Frédérique Vernel-Pauillac,

Tiffany R Hogan,

John W. Tapsall,

Cyrille Goarant

Publication year - 2009

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01104-08

Subject(s) - neisseria gonorrhoeae , quinolone , genotyping , biology , microbiology and biotechnology , neisseria , genotype , genetics , gene , antibiotics , bacteria

We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.

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