Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin | Zendy

Go Hirokawa | Zendy; Hideko Kaji | Zendy; Akira Kaji | Zendy

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Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin

Author(s) -

Go Hirokawa,

Hideko Kaji,

Akira Kaji

Publication year - 2006

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01096-06

Subject(s) - paromomycin , ribosome , ribosomal rna , initiation factor , biology , protein subunit , 30s , protein biosynthesis , biochemistry , microbiology and biotechnology , aminoglycoside , antibiotics , rna , gene

The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg(2+). Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.

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