Open AccessComplete Sequence of p07-406, a 24,179-Base-Pair Plasmid Harboring the bla VIM-7 Metallo-β-Lactamase Gene in a Pseudomonas aeruginosa Isolate from the United States
Author(s) -
H. Li,
Mark A. Toleman,
Peter M. Bennett,
Ronald N. Jones,
Timothy R. Walsh
Publication year - 2008
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01093-07
Subject(s) - transposable element , plasmid , biology , genetics , integron , gene , complete sequence , inverted repeat , insertion sequence , microbiology and biotechnology , genome
An outbreak involving aPseudomonas aeruginosa strain that was resistant to all tested antimicrobials except polymyxin B occurred in a hospital in Houston, TX. Previous studies on this strain showed that it possesses a novel mobile metallo-β-lactamase (MBL) gene, designatedbla VIM-7 , located on a plasmid (p07-406). Here, we report the complete sequence, annotation, and functional characterization of this plasmid. p07-406 is 24,179 bp in length, and 29 open reading frames were identified related to known or putatively recognized proteins. Analysis of this plasmid showed it to be comprised of four distinct regions: (i) a region of 5,200 bp having a Tn501 -like mercuric resistance (mer ) transposon upstream of the replication region; (ii) a Tn3 -like transposon carrying a truncated integron with abla VIM-7 gene and an insertion sequence inserted at the other end of this transposon; (iii) a region of four genes, upstream of the Tn3 -like transposon, possessing very high similarity to plasmid pXcB fromXanthomonas campestris pv.citri commonly associated with plants; (iv) a backbone sequence similar to the backbone structure of the IncP group plasmid Rms149, pB10, and R751. This is the first plasmid to be sequenced carrying an MBL gene and highlights the amelioration of DNA segments from disparate origins, most noticeably from plant pathogens.