Novel Ambler Class A β-Lactamase LAP-1 and Its Association with the Plasmid-Mediated Quinolone Resistance Determinant QnrS1 | Zendy

Laurent Poirel | Zendy; Vincent Cattoir | Zendy; Ana Luiza G. Soares | Zendy; Claude–James Soussy | Zendy; Patrice Nordmann | Zendy

Open Access

Novel Ambler Class A β-Lactamase LAP-1 and Its Association with the Plasmid-Mediated Quinolone Resistance Determinant QnrS1

Author(s) -

Laurent Poirel,

Vincent Cattoir,

Ana Luiza G. Soares,

Claude–James Soussy,

Patrice Nordmann

Publication year - 2006

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.01082-06

Subject(s) - plasmid , enterobacter cloacae , integron , biology , beta lactamase , clavulanic acid , microbiology and biotechnology , gene , escherichia coli , enterobacteriaceae , genetics , antibacterial agent , tazobactam , pseudomonas aeruginosa , bacteria , piperacillin , antibiotics , amoxicillin

The plasmid-mediated quinolone resistance determinant QnrS1 was identified in non-clonally relatedEnterobacter cloacae isolates in association with a transferable narrow-spectrum β-lactam resistance marker. Cloning experiments allowed the identification of a novel Ambler class A β-lactamase, named LAP-1. It shares 62 and 61% amino acid identity with the most closely related β-lactamases, TEM-1 and SHV-1, respectively. It has a narrow-spectrum hydrolysis of β-lactams and is strongly inhibited by clavulanic acid and sulbactam and, to a lesser extent, by tazobactam. Association of thebla LAP-1 gene with theqnrS1 gene was identified inE. cloacae isolates from France and Vietnam. These genes were plasmid located and associated with similar insertion sequences but were not associated withsul1 -type class 1 integrons, as opposed to theqnrA genes.

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