Open AccessMulticlonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil
Author(s) -
Caio Augusto Martins Aires,
Polyana Silva Pereira,
Carlos Felipe Machado de Araújo,
Thiago Pavoni Gomes Chagas,
Jane Cleide Ribeiro Oliveira,
Sibelle Nogueira Buonora,
Rodolpho Mattos Albano,
Ana Paula D’Alincourt Carvalho-Assef,
Marise Dutra Asensi
Publication year - 2017
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01048-16
Subject(s) - multilocus sequence typing , pulsed field gel electrophoresis , klebsiella pneumoniae , microbiology and biotechnology , biology , genotyping , amikacin , tigecycline , context (archaeology) , typing , genetics , genotype , antibiotics , gene , escherichia coli , paleontology
We characterized NDM-1-producing Klebsiella isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of bla NDM was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing Klebsiella pneumoniae was not associated with clonal expansion and appears to be associated with Tn 3000 .
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