Open Access3-Hydroxyphthalic Anhydride-Modified Chicken Ovalbumin Exhibits Potent and Broad Anti-HIV-1 Activity: a Potential Microbicide for Preventing Sexual Transmission of HIV-1
Author(s) -
Lin Li,
Lili He,
Suiyi Tan,
Xinhua Guo,
Hong Liang,
Zhi Qi,
Chungen Pan,
Xiuli An,
Shibo Jiang,
Shuwen Liu
Publication year - 2010
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.01046-09
Subject(s) - microbicide , ovalbumin , sexual transmission , gp41 , virology , entry inhibitor , microbicides for sexually transmitted diseases , human immunodeficiency virus (hiv) , biology , transmission (telecommunications) , pharmacology , virus , immunology , medicine , viral entry , viral replication , population , antigen , epitope , environmental health , health services , engineering , electrical engineering
Heterosexual transmission is the primary route by which women acquire human immunodeficiency virus (HIV)/AIDS. Thus, development of woman-controlled topical microbicides for prevention of sexual transmission of HIV is urgently needed. Here we report that 3-hydroxyphthalic anhydride-modified chicken ovalbumin (HP-OVA) exhibits potent antiviral activity against a broad spectrum of human immunodeficiency virus type 1 (HIV-1) isolates with different genotypes and biotypes. Its antiviral activity is correlated with the percentages of the chemically modified and unmodified lysines and arginines in OVA. HP-OVA inhibits HIV-1 fusion and entry through multiple mechanisms of action, including (i) blocking gp120 binding to CD4 and (ii) interfering with gp41 six-helix bundle formation. Because of the widespread availability and established safety profile of OVA, HP-OVA has good potential to be developed as an effective, safe, and affordable microbicide for prevention of HIV sexual transmission.